WB result of SV2A Recombinant Rabbit mAb
Primary antibody: SV2A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SH-SY5Y whole cell unboiled RIPA lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 83 kDa
Observed MW: 120-140 kDa
SV2A Recombinant Rabbit mAb (S-2096-2)
SV2A Recombinant Rabbit mAb (S-2096-2)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SV2A |
| Synonyms | Synaptic vesicle glycoprotein 2A; KIAA0736 |
| Immunogen | Synthetic Peptide |
| Location | Synapse |
| Accession | Q7L0J3 |
| Clone Number | S-2096-2 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | SH-SY5Y, mouse brain, rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
Background
Synaptic vesicle glycoprotein 2A (SV2A) is a transmembrane protein found in synaptic vesicles throughout the brain, playing a crucial role in neuronal function. It is involved in regulating neurotransmitter release by modulating the coupling between calcium entry and neurotransmitter release during exocytosis. SV2A also interacts with other synaptic proteins, such as synaptotagmin, and may have additional functions including the transport of galactose and interaction with extracellular matrix components like laminin. Notably, SV2A is the specific target of the anti-epileptic drug levetiracetam, which has led to increased interest in its role in epilepsy and other neurological disorders. Recent studies have also suggested that SV2A may have a mitochondrial localization and function, potentially acting as a fusion or fission factor, which could be relevant to the study of mitochondrial dysfunctions in diseases like Alzheimer’s.
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Western Blot
WB result of SV2A Recombinant Rabbit mAb
Primary antibody: SV2A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain unboiled RIPA lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 83 kDa
Observed MW: 110-140 kDa
WB result of SV2A Recombinant Rabbit mAb
Primary antibody: SV2A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain unboiled RIPA lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 83 kDa
Observed MW: 110-140 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human pancreas. Anti-SV2A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-SV2A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-SV2A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-SV2A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
