WB result of SUZ12 Recombinant Rabbit mAb
Primary antibody: CTCF Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: Caco-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 83 kDa
Observed MW: 90 kDa
SUZ12 Recombinant Rabbit mAb (S-1689-3)
SUZ12 Recombinant Rabbit mAb (S-1689-3)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SUZ12 |
| Synonyms | Polycomb protein SUZ12; Chromatin precipitated E2F target 9 protein (ChET 9 protein); Joined to JAZF1 protein; Suppressor of zeste 12 protein homolog; CHET9; JJAZ1; KIAA0160 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q15022 |
| Clone Number | S-1689-3 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC, ICFCM |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, MCF7, Caco-2, NIH/3T3, C6 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms |
| ICFCM | 1:50 | Ms |
Background
SUZ12, also known as Zeste 12 Homolog, is a protein encoded by the human SUZ12 gene and is a component of the Polycomb Repressive Complex 2 (PRC2). SUZ12 plays a significant role in epigenetic modification, particularly in Regulating tyrosine trimethylation at position 27 of histone H3. SUZ12 has been implicated in the proliferation and metastasis of various types of cancer. It is overexpressed in tumors such as lung, stomach, colorectal, and breast cancer, where it promotes tumor growth and invasion. In hepatocellular carcinoma, SUZ12 downregulation has been associated with the upregulation of tumor stem cell marker genes, suggesting its importance in tumorigenesis and cancer progression.
Picture
Picture
Western Blot
WB result of SUZ12 Recombinant Rabbit mAb
Primary antibody: CTCF Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 83 kDa
Observed MW: 90 kDa
WB result of SUZ12 Recombinant Rabbit mAb
Primary antibody: CTCF Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 83 kDa
Observed MW: 90 kDa
FC
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelling SUZ12 antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- SUZ12 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- SUZ12 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
