WB result of SUV39H1 Recombinant Rabbit mAb
Primary antibody: SUV39H1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Ramos whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HCT 116 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 45 kDa
SUV39H1 Recombinant Rabbit mAb (S-3563-3)
SUV39H1 Recombinant Rabbit mAb (S-3563-3)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SUV39H1 |
| Synonyms | Histone-lysine N-methyltransferase SUV39H1; Histone H3-K9 methyltransferase 1 (H3-K9-HMTase 1); Lysine N-methyltransferase 1A; Position-effect variegation 3-9 homolog; Suppressor of variegation 3-9 homolog 1 (Su(var)3-9 homolog 1); KMT1A; SUV39H |
| Immunogen | Synthetic Peptide |
| Location | Nucleus, Cell membrane |
| Accession | O43463 |
| Clone Number | S-3563-3 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Mk |
| Positive Sample | Ramos, HeLa, HCT 116, NIH/3T3, COS-7 |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Mk |
| ICC | 1:200 | Hu |
Background
SUV39H1 (Suppressor of Variegation 3-9 Homolog 1) is a critical histone methyltransferase enzyme that specifically catalyzes the di- and tri-methylation of lysine 9 on histone H3 (H3K9me2/3), a repressive epigenetic mark essential for the formation and maintenance of constitutive heterochromatin, particularly at pericentromeric regions and repetitive DNA sequences. As a member of the SET domain-containing protein family, SUV39H1 plays a pivotal role in preserving genomic stability by silencing transposable elements, preventing chromosomal rearrangements, and ensuring proper chromosome segregation during cell division; its function is tightly regulated through interactions with other chromatin-modifying complexes, including HP1 (Heterochromatin Protein 1), which binds to the H3K9me3 marks created by SUV39H1 to further compact chromatin structure. Dysregulation or mutation of SUV39H1 has been implicated in various pathological conditions, including cancer progression, where altered heterochromatin dynamics can lead to genomic instability and aberrant gene expression, as well as in aging processes, highlighting its significance as a key regulator of epigenetic landscape and cellular identity.
Picture
Picture
Western Blot
WB result of SUV39H1 Recombinant Rabbit mAb
Primary antibody: SUV39H1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 45 kDa
WB result of SUV39H1 Recombinant Rabbit mAb
Primary antibody: SUV39H1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 45 kDa
Immunocytochemistry
ICC shows positive staining in Ramos cells. Anti-SUV39H1 antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
