WB result of Survivin Recombinant Rabbit mAb
Primary antibody: Survivin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: Ramos whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Lane 5: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 17 kDa
Survivin Recombinant Rabbit mAb (S-2334-26)
Survivin Recombinant Rabbit mAb (S-2334-26)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Survivin |
| Synonyms | Baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor 4; Apoptosis inhibitor survivin; API4; IAP4; BIRC5 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cytoskeleton, Nucleus |
| Accession | O15392 |
| Clone Number | S-2334-26 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Mk |
| Positive Sample | MCF7, Jurkat, Ramos, HeLa, A549, COS-7 |
| Predicted Reactivity | Bv, Ct, Dg, Pg |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Mk |
| IHC-P | 1:200 | Hu |
| ICC | 1:500 | Hu |
Background
Survivin is a 16.5 kDa member of the inhibitor of apoptosis (IAP) family that uniquely integrates control of cell division and suppression of apoptosis by binding to caspases-3 and -7, thereby blocking the terminal execution phase of programmed cell death, while also associating with the chromosomal passenger complex at centromeres during mitosis to ensure accurate chromosome segregation; it is transcriptionally repressed by wild-type p53, strongly up-regulated in virtually every human cancer through PI3K/AKT, STAT3, β-catenin/TCF and HIF-1α pathways, and correlates with poor prognosis, resistance to chemo- and radiotherapy, and increased tumor recurrence, making it a high-value therapeutic target addressed by antisense oligonucleotides, dominant-negative mutants, small-molecule antagonists and immunotherapies that collectively seek to restore apoptotic sensitivity and inhibit unchecked proliferation in malignant cells.
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Picture
Western Blot
WB result of Survivin Recombinant Rabbit mAb
Primary antibody: Survivin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 17 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-Survivin antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-Survivin antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-Survivin antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-Survivin antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells (top panel) and low staining in MCF7 cells (below panel). Anti-Survivin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
