IHC shows positive staining in paraffin-embedded human lung. Anti-Surfactant Protein B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Surfactant Protein B Recombinant Rabbit mAb (SDT-435-18)
Surfactant Protein B Recombinant Rabbit mAb (SDT-435-18)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Surfactant Protein B |
| Synonyms | Pulmonary surfactant-associated protein B, SP-B, 18 kDa pulmonary-surfactant protein, 6 kDa protein, Pulmonary surfactant-associated proteolipid SPL(Phe) |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | P07988 |
| Clone Number | SDT-435-18 |
| Antibody Type | Rabbit mAb |
| Application | IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.25 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC | 1:1000 |
Background
Surfactant protein B is an essential lipid-associated protein found in pulmonary surfactant. Without it, the lung would not be able to inflate after a deep breath out [PMID: 19220077]. It rearranges lipid molecules in the fluid lining the lung so that tiny air sacs in the lung, called alveoli, can more easily inflate [PMID: 19220077].
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human lung cancer paracancerous tissue. Anti-Surfactant Protein B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
