Mouse splenocytes were isolated using biotinylated anti-mouse CD3 antibody. Subsequently, the cells were stained with anti-CD3 (clone: 17A2) Alexa Fluor®488, and the live cell population was gated for analysis.
Streptavidin Nanobeads
Streptavidin Nanobeads
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Product Details
Product Details
Product Specification
| Format | Streptavidin-conjugated dextran-coated iron oxide magnetic nanoparticles |
| Capacity |
1 mL magnetic beads: suitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs 0.5 mL magnetic beads: suitable for 5×108total cells, and can perform up to 50 sorting runs |
| Principle of Separation | Stain the cells with biotinylated primary antibodies or ligands. Subsequently, magnetically label the cells using Streptavidin Nanobeads. Then, load the cell suspension onto a separation column placed within the separator's magnetic field. The magnetically labeled cells are retained inside the column, while the unlabeled cells pass through it. After removing the column from the magnetic field, the magnetically retained cells can be eluted as the positively selected cell fraction. |
| Reactivity | Human, Mouse |
| Separation Method | Column-Based |
| Selection Strategy | Positive Selection |
| Stability & Storage | Store protected from light at 2–8°C; do not freeze. |
Background
Streptavidin NanobeadsDesigned to isolate cells based on surface markers labeled with biotinylated antibodies or ligands.
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Validation Data
