Streptavidin Donor Beads

Homogeneous Immuno Chemiluminescence Assay (HICA) is a homogeneous immunoassay method based on energy transfer between donor beads and acceptor beads at close proximity, resulting in luminescence.

Donor beads recognize Protein 1 (Tag1 label), while acceptor beads recognize Protein 2 (Tag2 label). When Protein 1 binds to Protein 2, the distance between the beads becomes less than 200 nm. Upon excitation at 680 nm, the donor beads generate singlet oxygen, which diffuses to the acceptor beads. The acceptor beads then undergo a redox reaction, emitting light at 615 nm. The signal intensity is directly proportional to the strength of the protein interaction.

This product features a simple operation process, requires no washing, and offers high speed and sensitivity. It is capable of detecting weak interactions.

【RequiredReagents】

【Assay Procedure for Reference】

【Performance Validation】

•Sample Preparation:

Use Universal Buffer 1 to pre-dilute Biotinylated Rabbit IgG (Bio-rIgG) to 15μg/mL (100nM) as the stock solution, then perform serial dilutions according to the following scheme:

•Detection Reagent Preparation:

•37℃ Incubation Mode Detection Results:

Maximum Signal:7151971

Minimum Signal:866

EC50= 0.195 nM

•Room Temperature Incubation Mode Detection Results:

Maximum Signal:3031800

Minimum Signal:389

EC50= 0.176 nM

1. This experiment is light-sensitive; perform all procedures under light-protected conditions. Preparation, sample loading, and incubation steps are recommended to be conducted under green light (illuminance below 100 LUX). 2. This product is compatible with multi-mode microplate readers equipped with Alpha detection modules. 3. Vortex thoroughly before use. Alternatively, briefly centrifuge (2000×g, 5–10 seconds) to ensure complete sample retrieval. 4. It is recommended to use the accompanying dilution buffer for reagent preparation and sample dilution. If additional components are required, they may be directly added to this buffer. 5. To ensure comparability of experimental data across different batches, strictly control incubation temperature and duration. 6. Avoid bubble formation during sample loading.