Linearity
Three different mouse spleen cells concentrations, 8 replicates, 1 batch. Data presented the mean spot count, range, and coefficient of variation corresponding to the three cell concentrations.
StepSlim Mouse IL-5 (HRP) ELISpot Kit
StepSlim Mouse IL-5 (HRP) ELISpot Kit
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$365 USD
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Product Details
Product Details
Product Specification
| Antigen | IL-5 |
| Antibody Type | Recombinant mAb |
| Reactivity | Mouse |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8℃ as supplied. |
Kit
| Precision | Intra-assay: 15.76%； Inter-assay: 17.14% |
| Sample type | Spleen cells |
| Assay type | Sandwich (Qualitative) |
| Assay time | 18-24 hours |
| Species reactivity | Mouse |
| Plate | Strip |
Background
Mouse Interleukin-5 (IL-5) is a Th2-derived cytokine critical for eosinophil biology. Produced primarily by activated T helper 2 cells and group 2 innate lymphoid cells (ILC2s) in mice, it signals through a heterodimeric receptor (IL-5Rα/βc) to regulate eosinophil differentiation, survival, and activation. This 45-kDa homodimeric glycoprotein drives allergic inflammation and anti-helminth immunity by promoting eosinophil migration to tissues and stimulating immunoglobulin production in B-1 cells. Its pivotal role in eosinophil-mediated pathologies makes it a key therapeutic target in murine asthma models and parasitic infections.
Picture
Picture
Elispot
Precision
Stimulate 4×10⁵ cells/well of mouse spleen cells with Con A for 20 hours at 37 °C in a 5% CO₂ incubator. Repeat across 8 wells in 3 batches to evaluate inter-batch and intra-batch precision. Data presented include mean spot count, range, and coefficient of variation.
Diagram of spots
Stimulate 4×10⁵ cells/well of mouse spleen cells with Con A. After 20 hours of stimulation at 37 °C in a 5% CO₂ incubator, incubate with HRP-labeled detection antibody at room temperature for 2 hours. Add substrate for color development, followed by image analysis and spot counting.
