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StepSlim Mouse IFN-γ (ALP) Plus ELISpot Kit

StepSlim Mouse IFN-γ (ALP) Plus ELISpot Kit

Catalog Number: S0P1019 Reactivity: Mouse Conjugation: Brand: Starter
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Regular price $400 USD
Regular price Sale price $400 USD
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Product Details

Product Specification


Antigen IFN-γ
Antibody Type Recombinant mAb
Reactivity Mouse
Purification Protein A
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8℃ as supplied.

Kit


Precision Intra-assay: 2.93%;
Inter-assay: 3.91%
Sample type Spleen cells
Assay type Sandwich (Qualitative)
Assay time 18-24 hours
Species reactivity Mouse
Plate Strip

Background

Mouse IFN-γ, or Interferon-gamma, is a crucial cytokine primarily produced by natural killer (NK) cells and T lymphocytes, particularly Th1 cells and cytotoxic T cells. It plays a pivotal role in the immune response, acting as a key mediator in both innate and adaptive immunity. IFN-γ is known for its potent antiviral, immunoregulatory, and anti-tumor properties. It enhances the presentation of antigens by upregulating major histocompatibility complex (MHC) class I and II molecules on antigen-presenting cells, thereby facilitating a more robust immune response. Additionally, IFN-γ activates macrophages, increasing their ability to phagocytose and destroy pathogens. It also promotes the differentiation of naive T cells into Th1 cells, further amplifying the immune response. In research, mouse IFN-γ is widely used to study immune responses, inflammatory diseases, and host-pathogen interactions, providing valuable insights into the mechanisms of immune regulation and potential therapeutic strategies.

Picture

Elispot

Linearity
Five different mouse spleen cells concentrations, 8 replicates, 1 batch. Data presented the mean spot count, range, and coefficient of variation corresponding to the five cell concentrations.

Precision
Stimulate 2×10⁵ cells/well of mouse spleen cells with Con A for 22 hours at 37°C in a 5% CO₂ incubator. Repeat across 8 wells in 3 batches to evaluate inter-batch and intra-batch precision. Data presented include mean spot count, range, and coefficient of variation.

Diagram of spots
Stimulate 2×10⁵ cells/well of mouse spleen cells with Con A. After 22 hours of stimulation at 37°C in a 5% CO₂ incubator, incubate with ALP-labeled detection antibody at room temperature for 2 hours. Add substrate for color development, followed by image analysis and spot counting.

Protocol Diagram