STEAP1+STEAP1B1+STEAP1B2 Recombinant Rabbit mAb (S-3226-137)

Catalog Number: S0B6883 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25µl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	STEAP1+STEAP1B1+SPEAP1B2
Synonyms	STEAP1 protein; PRSS24; STEAP
Location	Endosome, Cell membrane
Accession	Q9UHE8
Clone Number	S-3226
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu
Positive Sample	LNCap, 22Rv1
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu
IHC-P	1:2000	Hu

Background

STEAP1, STEAP1B1, and STEAP1B2 (also known as STEAP1B) belong to the STEAP (Six-Transmembrane Epithelial Antigen of the Prostate) protein family. A common feature of these proteins is that they all contain a C-terminal domain homologous to yeast ferric reductase (FRE), which includes a b-type heme prosthetic group—a core component essential for their potential metal reductase function. Among them, STEAP1 is the most extensively studied member of the family. It is highly upregulated on the cell surface of various human cancers (such as prostate cancer and lung cancer) and is therefore regarded as a highly promising target for cancer therapy. However, unlike other family members (STEAP2–4), STEAP1 lacks an intracellular NADPH-binding domain and thus does not possess independent ferric reductase activity. Cryo-electron microscopy structures have revealed that STEAP1 exists as a trimer, and it is hypothesized that it functions by forming heterotrimeric complexes with other family members such as STEAP2 or STEAP4, which provide the NADPH-binding domain, thereby cooperating to complete the reduction of metal ions. Meanwhile, STEAP1B1 and STEAP1B2, as homologs (or truncated homologs) of STEAP1, also possess the ferric reductase-like transmembrane domain. However, their specific biological functions, tissue distribution, and precise mechanisms of interaction with STEAP1 remain to be further elucidated. Current studies suggest that they may have synergistic or compensatory functional relationships with STEAP1.

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Western Blot

WB result of STEAP1+STEAP1B1+SPEAP1B2 Recombinant Rabbit mAb
Primary antibody: STEAP1+STEAP1B1+SPEAP1B2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-3 whole cell lysate 20 µg
Lane 2: DU 145 whole cell lysate 20 µg
Lane 3: LNCaP whole cell lysate 20 µg
Lane 4: 22Rv1 whole cell lysate 20 µg
Negative control: PC-3 whole cell lysate; DU 145 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 35 kDa

WB result of STEAP1+STEAP1B1+SPEAP1B2 Recombinant Rabbit mAb
Primary antibody: STEAP1+STEAP1B1+SPEAP1B2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293F transfected with human STEAP2 expression vector containing a His-tag whole cell lysate 20 µg
Lane 2: 293F transfected with human STEAP3 expression vector containing a His-tag whole cell lysate 20 µg
Lane 3: 293F transfected with human STEAP4 expression vector containing a His-tag whole cell lysate 20 µg
Lane 4: 293F transfected with human STEAP1B1 expression vector containing a His-tag whole cell lysate 20 µg
Lane 5: 293F transfected with human STEAP1B2 expression vector containing a His-tag whole cell lysate 20 µg
Lane 6: 293F transfected with human STEAP1 expression vector containing a His-tag whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 35 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human prostatic cancer (case 1). Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human prostatic cancer (case 2). Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human prostatic cancer (case 3). Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human prostatic cancer (case 4). Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human breast cancer. Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human endometrial cancer. Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human STEAP1 transfected 293F cell pellets. Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human STEAP1B1 transfected 293F cell pellets. Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human STEAP1B2 transfected 293F cell pellets. Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human STEAP2 transfected 293F cell pellets. Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human STEAP3 transfected 293F cell pellets. Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human STEAP4 transfected 293F cell pellets. Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded vector transfected 293F cell pellets. Anti-STEAP1+STEAP1B1+SPEAP1B2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.