STEAP1 Recombinant Rabbit mAb (S-3613-107)

Catalog Number: S0B6880 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25µl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	STEAP1
Synonyms	STEAP1 protein; PRSS24; STEAP
Immunogen	Synthetic Peptide
Location	Endosome, Cell membrane
Accession	Q9UHE8
Clone Number	S-3613-107
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu
Positive Sample	LNCap, 22Rv1
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:500-1:1000	Hu
IHC-P	1:2000	Hu

Background

STEAP1 (Six-Transmembrane Epithelial Antigen of the Prostate 1) is a metalloreductase protein that plays a crucial role in cellular iron and copper homeostasis by facilitating the reduction of Fe³⁺ to Fe²⁺ and Cu²⁺ to Cu⁺, thereby enabling the transport of these essential metal ions across cell membranes. Primarily expressed in prostate epithelium, STEAP1 has gained significant attention as a promising therapeutic target in oncology, particularly for prostate cancer, where it is frequently overexpressed and serves as a cell surface antigen for antibody-drug conjugates and CAR-T cell therapies.

Picture

Western Blot

WB result of STEAP1 Recombinant Rabbit mAb
Primary antibody: STEAP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-3 whole cell lysate 20 µg
Lane 2: DU 145 whole cell lysate 20 µg
Lane 3: LNCaP whole cell lysate 20 µg
Lane 4: 22Rv1 whole cell lysate 20 µg
Negative control: PC-3 whole cell lysate; DU 145 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 35 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human STEAP1 transfected 293F cell pellets. Anti-STEAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human STEAP1B1 transfected 293F cell pellets. Anti-STEAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human STEAP1B2 transfected 293F cell pellets. Anti-STEAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human STEAP2 transfected 293F cell pellets. Anti-STEAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human STEAP3 transfected 293F cell pellets. Anti-STEAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human STEAP4 transfected 293F cell pellets. Anti-STEAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded vector transfected 293F cell pellets. Anti-STEAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded PC-3 cell pellets. Anti-STEAP1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.