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STAT6 GST Tag Protein, Human

STAT6 GST Tag Protein, Human

Catalog Number: UA085028 Brand: UA BIOSCIENCE
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$370 USD
$370 USD
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Product Details

Product Specification


Species Human
Synonyms STAT6C, STAT6B, interleukin-4 induced, IL-4-STAT, D12S1644
Accession P42226-1
Amino Acid Sequence

Trp123-Thr658 with GST Tag at the N-Terminus
Expression System E. coli
Molecular Weight 70-100kDa (Reducing)
Purity ＞80% by SDS-PAGE
Conjugation Unconjugated
Tag GST Tag
Physical Appearance Liquid
Storage Buffer

50mM Tris, 200mM NaCl, 1mM DTT, 1mM EDTA, 20% Glycerol, pH7.5
Stability & Storage

Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
Reference

1.Karpathiou G, Papoudou-Bai A, Ferrand E, Dumollard JM, Peoc'h M. STAT6: A review of a signaling pathway implicated in various diseases with a special emphasis in its usefulness in pathology. Pathol Res Pract. 2021 Jul;223:153477.
2.Chao H, Zheng L, Hsu P, He J, Wu R, Xu S, Zeng R, Zhou Y, Ma H, Liu H, Tang Q. IL-13RA2 downregulation in fibroblasts promotes keloid fibrosis via JAK/STAT6 activation. JCI Insight. 2023 Mar 22;8(6):e157091.

Background

STAT6 (Signal Transducer and Activator of Transcription 6) is a transcription factor that plays a pivotal role in the cellular response to interleukin-4 (IL-4). It is activated through IL-4-induced tyrosine phosphorylation. Its primary function is to mediate most of IL-4's effects, including promoting cell survival by inducing the expression of the anti-apoptotic gene BCL2L1/BCL-X(L). To activate transcription, STAT6 requires interaction with coactivator proteins like p300 and CBP. A key physiological characteristic is its direct interaction with NF-κB, which underlies the synergistic transcriptional activation observed between the IL-4 and NF-κB signaling pathways. Clinically, its central role in IL-4-mediated responses makes it a molecule of interest in immune-related processes.

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Bioactivity

The STAT6 activity was detected using TR-FRET technology. The reaction was performed by incubating the varying concentrations of AK-1690 and STAT6 protein at room temperature for 10 min, adding substrate and the beads at room temperature for 60 min, then reading ratio 665/620 with BMG.

SDS-PAGE

2μg(R:reducing condition, N:non-reducing condition).