WB result of Stat1 Rabbit mAb
Primary antibody: Stat1 Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 5 µg
Lane 2: HeLa whole cell lysate 5 µg
Lane 3: MCF7 whole cell lysate 5 µg
Lane 4: A549 whole cell lysate 5 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 87 kDa
Observed MW: 84, 91 kDa
Stat1 Recombinant Rabbit mAb (S-559-91)
Stat1 Recombinant Rabbit mAb (S-559-91)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Stat1 |
| Synonyms | Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84 |
| Location | Cytoplasm, Nucleus |
| Clone Number | S-559-91 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IP |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20°C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IP | 1:50 |
Background
Signal transducer and activator of transcription 1 (STAT1) is a transcription factor which in humans is encoded by the STAT1 gene. It is a member of the STAT protein family. All STAT molecules are phosphorylated by receptor associated kinases, that causes activation, dimerization by forming homo- or heterodimers and finally translocate to nucleus to work as transcription factors. Specifically STAT1 can be activated by several ligands such as Interferon alpha (IFNα), Interferon gamma (IFNγ), Epidermal Growth Factor (EGF), Platelet Derived Growth Factor (PDGF), Interleukin 6 (IL-6), or IL-27.
Picture
Picture
Western Blot
WB result of Stat1 Rabbit mAb
Primary antibody: Stat1 Rabbit mAb at 1/1000 dilution
Lane 1: mouse heart lysate 5 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 87 kDa
Observed MW: 84, 91 kDa
WB result of Stat1 Rabbit mAb
Primary antibody: Stat1 Rabbit mAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 87 kDa
Observed MW: 91 kDa
IP
Stat1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Stat1 in 0.4 mg 293T whole cell lysate.
Western blot was performed on the immunoprecipitate using Stat1 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: 293T whole cell lysate 20 µg (Input)
Lane 2: Stat1 Rabbit mAb IP in 293T whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in 293T whole cell lysate
Predicted MW: 87 kDa
Observed MW: 84, 91 kDa
