WB result of StAR Recombinant Rabbit mAb
Primary antibody: StAR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Ramos whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate; Ramos whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 25 kDa
This blot was developed with high sensitivity substrate
StAR Recombinant Rabbit mAb (S-2520-24)
StAR Recombinant Rabbit mAb (S-2520-24)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | StAR |
| Synonyms | Steroidogenic acute regulatory protein, mitochondrial; START domain-containing protein 1 (StARD1); STARD1; STAR |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion |
| Accession | P49675 |
| Clone Number | S-2520-24 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Rt |
| Positive Sample | K562 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:500 | Hu, Rt |
Background
StAR (Steroidogenic Acute Regulatory) protein is a 37-kDa mitochondrial phosphoprotein that catalyzes the rate-limiting step in steroid hormone biosynthesis by ferrying cholesterol from the outer to the inner mitochondrial membrane; upon tropic hormone stimulation its N-terminal 25-amino-acid mitochondrial targeting sequence is cleaved to yield the 30-kDa mature form, and mutations in the STAR gene cause the potentially fatal lipoid congenital adrenal hyperplasia in humans.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-StAR antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human adrenal gland. Anti-StAR antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pheochromocytoma. Anti-StAR antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-StAR antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-StAR antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
