Human peripheral blood cells were labeled with Anti-Human CD15 antibody at 1/500 dilution (1 μg). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. The cells were resuspended in either STARTER Staining Buffer (FBS) (left panel) or Supplier B Staining Buffer (right panel). Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Staining Buffer (FBS)
Staining Buffer (FBS)
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$85 USD
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Product Details
Product Details
Product Specification
| Concentration | 1× |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
Staining Buffer (FBS) is an antibody diluent and cell wash buffer optimized for immunofluorescent staining of viable or fixed single‑cell suspensions. It contains FBS (heat-inactivated) as a protein carrier to minimize non‑specific binding of antibodies and fluorochromes to target cells. The buffer also includes sodium azide (NaN₃), a metabolic inhibitor, which prevents patching and capping of cell surface antigens. Additionally, sodium azide, in combination with cold temperature maintenance, inhibits antigen redistribution (e.g., shedding or internalization) induced by antibody crosslinking, thereby preserving the fluorescent signal intensity of stained cells during subsequent flow cytometric or fluorescence microscopy analysis. To avoid interference in biotin/avidin‑based indirect staining protocols, the buffer is formulated without biotin, calcium, magnesium, and EDTA.
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