SSEA4 Recombinant Mouse mAb (Alexa Fluor® 647 Conjugate) (S-R541)

Flow cytometric analysis of SSEA4 expression on NTERA-2 cells. Cells from the NTERA-2 (Right) or HeLa (Human cervix adenocarcinoma epithelial cell, Left) was stained with either Alexa Fluor® 647 Rabbit IgG Isotype Control (Black line histogram) or SDT SSEA4 Recombinant Mouse mAb (Alexa Fluor® 647 Conjugate) (Red line histogram) at 1/320 (0.25 μg), cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.

ICC shows positive staining in NTERA-2 cells. Anti-SSEA4 (Alexa Fluor® 647 Conjugate) antibody was used at 1/100 dilution (meganta) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

ICC shows negative staining in HeLa cells. Anti- SSEA4 (Alexa Fluor® 647 Conjugate) antibody was used at 1/100 dilution and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).