The Src activity was detected using HTRF technology. The reaction was performed by incubating the Src protein, ATP and substrate at 25℃ for 60 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
SRC Protein
SRC Protein
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Product Details
Product Details
Product Specification
| Species | Human |
| Accession | P12931-1 |
| Amino Acid Sequence | M1-L536(end), with N-terminal GST tag |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 86457.0 |
| Purity | >90% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | GST Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50mM Tris, 150mM NaCl, 1mM DTT, 10% Glycerol, 0.05% Brij35, pH7.5 |
| Stability & Storage | -80℃ |
Picture
Picture
Bioactivity
SDS-PAGE
M MW Standard
1 1 μg Src
2 3 μg Src
