WB result of SPTB Recombinant Rabbit mAb
Primary antibody: SPTB Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 246 kDa
Observed MW: 280 kDa
SPTB Recombinant Rabbit mAb (S-755-63)
SPTB Recombinant Rabbit mAb (S-755-63)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SPTB |
| Synonyms | Spectrin beta chain, erythrocytic; Beta-I spectrin; SPTB1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cytoskeleton |
| Accession | P11277 |
| Clone Number | S-755-63 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | SH-SY5Y, mouse spleen, mouse brain, rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
SPTB (Spectrin Beta Chain, Erythrocytic) is one of the key proteins in the erythrocyte skeleton and belongs to the spectrin family. It is mainly composed of multiple spectrin repeating units, which form a flexible α-helical structure, giving SPTB a high degree of elasticity and mechanical stability. SPTB binds to α-spectrin (SPTA1) to form a heterodimer, which is further assembled into a tetramer, forming the main reticular structure of the erythrocyte skeleton, maintaining the morphology and mechanical strength of erythrocytes, enabling them to withstand shear forces in blood circulation. In addition, SPTB also stabilizes the cell membrane and regulates the mechanical properties of the cell membrane by interacting with other skeleton proteins such as ankyrin, actin, and protein 4.1. SPTB gene mutations can lead to hemolytic anemia diseases such as hereditary spherocytosis because they affect the integrity and function of the erythrocyte skeleton. SPTB is also expressed in non-erythrocytes and participates in processes such as cell morphology maintenance, cell migration, and signal transduction.
Picture
Picture
Western Blot
WB result of SPTB Recombinant Rabbit mAb
Primary antibody: SPTB Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse spleen lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 246 kDa
Observed MW: 280 kDa
WB result of SPTB Recombinant Rabbit mAb
Primary antibody: SPTB Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 246 kDa
Observed MW: 280 kDa
Immunocytochemistry
ICC shows positive staining in SH-SY5Y cells. Anti- SPTB antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
