Spike His Tag Protein, SARS-CoV-2(R682S,R685S,K986P&V987P)
Spike His Tag Protein, SARS-CoV-2(R682S,R685S,K986P&V987P)
Product Details
Product Details
Product Specification
| Species | SARS-CoV-2 |
| Synonyms | 2019 nCOV Spike S蛋白,S protein,Spike glycoprotein,S glycoprotein,COVID-19 |
| Accession | P0DTC2 |
| Amino Acid Sequence | Val16-Lys1211(R682S, R685S, K986P&V987P) |
| Expression System | HEK293 |
| Molecular Weight | 130-180kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, 5% trehalose, pH7.4. |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Yuejun Shi, Jiale Shi, Limeng Sun, Yubei Tan, Gang Wang, Fenglin Guo, Guangli Hu, Yanan Fu, Zhen F Fu, Shaobo Xiao.Insight into vaccine development for Alpha-coronaviruses based on structural and immunological analyses of spike proteins. J Virol. 2021 Mar 10;95(7):e02284-20. Epub 2021 Jan 7. |
Background
Coronaviruses that infect humans belong to theAlpha-coronavirus (including HCoV-229E) and Beta-coronavirus (includingSARS-CoV and SARS-CoV-2) genera. In particular, SARS-CoV-2 is currently a majorthreat to public health worldwide. The spike (S) homotrimers bind to theirreceptors via the receptor-binding domain (RBD), which is a major target toblock viral entry.our results reveal different vaccine strategies for coronaviruses, andS-trimer is better than RBD as a target for vaccine developmentin Alpha-coronavirus Our findings will provide important implicationsfor future development of coronavirus vaccines. Outbreak of coronaviruses,especially SARS-CoV-2, poses a serious threat to global public health.Development of vaccines to prevent the coronaviruses that can infect humans hasalways been a top priority. Coronavirus spike (S) protein is considered as amajor target for vaccine development. Currently, structural studies have shownthat Alpha-coronavirus (HCoV-229E)and Beta-coronavirus (SARS-CoV and SARS-CoV-2) RBDs are in"lying" and "standing" states in the prefusion S-trimerstructure. Here, we evaluated the ability of S-trimer and RBD to induceneutralizing antibodies among these coronaviruses. Our results showed that theS-trimer and RBD are both candidates for subunit vaccinesin Beta-coronavirus (SARS-CoV and SARS-CoV-2) with a RBD"standing" state. However,for Alpha-coronavirus (HCoV-229E) with a RBD "lying" state,the S-trimer may be more suitable for subunit vaccines than the RBD. Ourresults will provide novel ideas for the development of vaccines targeting Sprotein in the future.
