WB result of SPARCL1 Recombinant Rabbit mAb
Primary antibody: SPARCL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 75 kDa
Observed MW: 130 kDa
SPARCL1 Recombinant Rabbit mAb (S-3326-27)
SPARCL1 Recombinant Rabbit mAb (S-3326-27)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SPARCL1 |
| Synonyms | SPARC-like protein 1; High endothelial venule protein (Hevin); MAST 9 |
| Immunogen | Recombinant Protein |
| Location | Secreted, Extracellular matrix |
| Accession | Q14515 |
| Clone Number | S-3326-27 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Raji, mouse brain, mouse cerebellum, rat brain |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:100 | Hu |
Background
SPARCL1 (secreted protein acidic and rich in cysteine-like 1) is a matricellular heparin-binding glycoprotein of the SPARC family that is secreted by quiescent endothelial cells, astrocytes and other stromal cells, where it functions as a context-dependent modulator of cell–matrix interactions, antagonizing angiogenesis by blocking endothelial proliferation and migration via its N-terminal acidic domain, stabilizing vessel integrity and limiting vascular permeability in colitis and other inflammatory states, while in the brain it promotes excitatory synaptogenesis and astrocyte differentiation, and in cancer it frequently acts as a tumor suppressor whose down-regulation accelerates invasion and metastasis yet, conversely, in gliomas or under viral injury its up-regulation can enhance inflammation by shifting macrophages toward a pro-inflammatory M1 phenotype through TLR4 activation, altogether illustrating a pleiotropic protein that integrates extracellular cues to orchestrate vascular, neuronal and immune homeostasis.
Picture
Picture
Western Blot
WB result of SPARCL1 Recombinant Rabbit mAb
Primary antibody: SPARCL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse kidney lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Lane 3: mouse cerebellum lysate 20 µg
Negative control: mouse kidney lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 75 kDa
Observed MW: 130 kDa
WB result of SPARCL1 Recombinant Rabbit mAb
Primary antibody: SPARCL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 75 kDa
Observed MW: 130 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-SPARCL1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-SPARCL1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
