WB result of Sp7/Osterix Recombinant Rabbit mAb
Primary antibody: Sp7/Osterix Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Saos-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45 kDa
Observed MW: 45, 50 kDa
Sp7/Osterix Recombinant Rabbit mAb, PBS Only (SDT-3238-16)
Sp7/Osterix Recombinant Rabbit mAb, PBS Only (SDT-3238-16)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Sp7/Osterix |
| Synonyms | Transcription factor Sp7; Zinc finger protein osterix; OSX |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q8TDD2 |
| Clone Number | SDT-3238-16 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Saos-2, mouse articular cartilage, mouse embryo |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS |
| Stability & Storage | 12 months from date of receipt / reconstitution, 4 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
| IF | 1:1000 | Ms, Rt |
Background
Sp7/Osterix is a ~45 kDa zinc-finger transcription factor of the Sp/KLF family that is indispensable for osteoblast lineage commitment and bone formation, acting downstream of Runx2 and cooperatively with BMP-6 to drive expression of osteogenic genes such as COL1A1, ALP, OCN, SATB2 and SOST, while its own transcription is induced by BMP-2, IGF-I and PTH and its activity is tuned by Akt- and p38 MAPK-mediated phosphorylation, ubiquitin–proteasome degradation and autoregulation via an OSX-bound tandem repeat in its promoter, and genetic or regulatory disruption of this short-lived nuclear protein underlies skeletal disorders including osteogenesis imperfecta and osteoporosis.
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Picture
Western Blot
WB result of Sp7/Osterix Recombinant Rabbit mAb
Primary antibody: Sp7/Osterix Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse articular cartilage lysate 20 µg
Lane 3: mouse embryo lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45 kDa
Observed MW: 45, 50 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human chondroma. Anti-Sp7/Osterix antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded normal mouse E14 embryo. Anti-Sp7/Osterix antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded normal mouse E14 embryo. Anti-Sp7/Osterix antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded normal rat E14 embryo. Anti-Sp7/Osterix antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded normal rat E14 embryo. Anti-Sp7/Osterix antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in Saos2 cells. Anti- Sp7/Osterix antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse E14 embryo. Anti- Sp7/Osterix antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat E14 embryo. Anti- Sp7/Osterix antibody was used at 1/1000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
