SP100 Rabbit Polyclonal Antibody

Catalog Number: S0B60514 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

Quantity:

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Product Details

Product Specification

Host	Rabbit
Antigen	SP100
Immunogen	Synthetic Peptide
Location	Cytoplasm, Nucleus
Accession	P23497
Antibody Type	Polyclonal antibody
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu
Positive Sample	A431, HT-1080, Raji
Purification	Immunogen Affinity
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied.

Dilution

application	dilution	species
WB	1:500-1:1000	Hu
IHC-P	1:500	Hu

Background

The SP100 is a multifunctional protein primarily located within nuclear PML bodies (Promyelocytic Leukemia Protein bodies) and is a key component of these subnuclear structures. It exhibits a wide range of functions, acting as either a transcriptional co-activator or co-repressor in gene regulation, and through its interaction with HP1 family heterochromatin proteins, it links PML bodies to chromatin regions, thereby participating in chromatin dynamics and gene expression regulation. Physiologically, SP100 is involved in the regulation of cell growth, differentiation, and apoptosis, as well as in telomere maintenance, DNA damage response, and antiviral defense. Additionally, SP100 is an important autoantigen, and its antibodies serve as a critical serological marker for primary biliary cholangitis (PBC), an autoimmune disease. Furthermore, studies have revealed that SP100 plays complex and contradictory roles in various tumors, where changes in its expression levels are closely associated with the initiation, progression, and patient prognosis of different cancers, suggesting potential dual functions as either a tumor promoter or a tumor suppressor.

Picture

Western Blot

WB result of SP100 Rabbit Polyclonal Antibody
Primary antibody: SP100 Rabbit Polyclonal Antibody at 1/1000 dilution
Lane 1: HEK-293T whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: HT-1080 whole cell lysate 20 µg
Negative control: 293T whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 100 kDa
Observed MW: 60-80kDa

WB result of SP100 Rabbit Polyclonal Antibody
Primary antibody: SP100 Rabbit Polyclonal Antibody at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 100 kDa
Observed MW: 80 kDa
This blot was developed with high sensitivity substrate

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon. Anti-SP100 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human kidney. Anti-SP100 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human placenta. Anti-SP100 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.