WB result of SOX2 Recombinant Rabbit mAb
Primary antibody: SOX2 Recombinant Rabbit mAb at 1/2000 dilution
Lane 1: NCCIT whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 36 kDa
SOX2 Recombinant Rabbit mAb (S-5000)
SOX2 Recombinant Rabbit mAb (S-5000)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SOX2 |
| Synonyms | Transcription factor SOX-2 |
| Location | Nucleus |
| Accession | P48431 |
| Clone Number | S-5000 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | NCCIT, F9, C6 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:2000 | Hu |
| ICC | 1:100 | Hu |
Background
SOX2 (SRY-related HMG-box transcription factor 2) is a transcription factor belonging to the B subgroup of the SOX (SRY-related HMG-box) family, playing an indispensable and critical role in embryonic development and cell fate determination. Its core function is to recognize and bind specific DNA sequence motifs through its highly conserved HMG domain, thereby activating or repressing the transcription of downstream target genes. It exerts a central regulatory role in maintaining the pluripotency and self-renewal capacity of embryonic stem cells and pluripotent stem cells—forming a complex regulatory network with core transcription factors such as OCT4 and NANOG to collectively maintain stem cell "stemness" and inhibit differentiation. During normal development, SOX2 is not only essential for early embryonic development, neuroectoderm formation, and neural stem cell maintenance, but also participates in the development and maturation of multiple organs including the eye, ear, lung, and esophagus. However, while SOX2 expression is restricted in adult tissues, it is aberrantly overexpressed or dysregulated in various malignancies, such as glioblastoma, small-cell lung cancer, esophageal squamous cell carcinoma, breast cancer, and prostate cancer. Its abnormal activation is often closely associated with enhanced tumor aggressiveness, therapeutic resistance, and poor prognosis, primarily driving malignant tumor progression by promoting cancer stem cell properties, epithelial–mesenchymal transition (EMT), and anti-apoptotic capacity. Furthermore, SOX2 serves as one of the reprogramming factors (together with OCT4, KLF4, and c-MYC, constituting the Yamanaka factors) capable of inducing somatic cells into induced pluripotent stem cells (iPSCs), a discovery that has brought revolutionary breakthroughs in regenerative medicine and disease modeling. Given its dual critical roles in stemness maintenance and tumorigenesis, SOX2 has become a hotspot molecule in stem cell biology, developmental biology, and oncology research. Meanwhile, ongoing exploration of its regulatory mechanisms and targeted intervention strategies continues to advance, providing important directions for the development of novel anticancer and regenerative medicine therapies.
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Western Blot
WB result of SOX2 Recombinant Rabbit mAb
Primary antibody: SOX2 Recombinant Rabbit mAb at 1/2000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: F9 whole cell lysate 20 µg
Negative control: NIH/3T3 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 36 kDa
WB result of SOX2 Recombinant Rabbit mAb
Primary antibody: SOX2 Recombinant Rabbit mAb at 1/2000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 36 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human glioma. Anti-SOX2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-SOX2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-SOX2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human seminoma. Anti-SOX2 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in NCCIT cells. Anti-SOX2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in F9 cells. Anti-SOX2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Negative control: ICC shows negative staining in NIH/3T3 cells. Anti-SOX2 antibody was used at 1/100 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
