WB result of SOX17 Recombinant Rabbit mAb
Primary antibody: SOX17 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SK-OV-3 whole cell lysate 20 µg
Lane 2: OVCAR-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 50 kDa
SOX17 Recombinant Rabbit mAb (SDT-1803-48)
SOX17 Recombinant Rabbit mAb (SDT-1803-48)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SOX17 |
| Synonyms | Transcription factor SOX-17 |
| Immunogen | Recombinant Protein |
| Location | Nucleus |
| Accession | Q9H6I2 |
| Clone Number | SDT-1803-48 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Positive Sample | SK-OV-3, OVCAR-3 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:1000 | Hu |
| ICC | 1:500 | Hu |
Background
SOX17 protein, a member of the SOX (SRY-related HMG-box) family of transcription factors, is crucial for the regulation of embryonic development and cell fate determination. It plays a key role in endoderm development, cardiovascular system formation, maintenance of fetal hematopoietic stem cells, and postnatal angiogenesis. SOX17 also functions as a transcriptional regulator that binds to specific DNA sequences and modulates transcriptional regulation via WNT3A, inhibiting Wnt signaling and promoting the degradation of activated CTNNB1. Furthermore, it has been implicated in cancer, particularly as a mutated cancer driver gene in endometrial carcinoma, with missense mutations potentially converting SOX17 into a pluripotency-inducing factor and altering its DNA binding preferences and gene expression programs. SOX17 is also considered to act as a tumor suppressor through the attenuation of WNT/β-catenin signaling in various cancer types.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-SOX17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human ovary. Anti-SOX17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-SOX17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human seminoma. Anti-SOX17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human testis. Anti-SOX17 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in SK-OV-3 cells. Anti- SOX1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
