SOCS2 Recombinant Rabbit mAb (S-4956-21)

Catalog Number: S0B60513 Application: WB, IHC-P Reactivity: Hu, Ms, Rt, Mk Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	SOCS2
Immunogen	Synthetic Peptide
Location	Cytoplasm
Accession	O14508
Clone Number	S-4956-21
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt, Mk
Positive Sample	K-562, A-204, KU812, Caco-2, mouse brain, PC-12, rat brain, COS-7
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt, Mk
IHC-P	1:200	Hu

Background

SOCS2 is an important member of the SOCS family. As a classic negative feedback regulatory protein, it plays a critical inhibitory role in the JAK/STAT signaling pathway. The core function of SOCS2 is to specifically recognize and bind to phosphorylated tyrosine sites on target proteins through its SH2 domain, and it then serves as the substrate-recognition component of the E3 ubiquitin ligase complex (CRL5), mediating the ubiquitination and proteasomal degradation of target proteins. Studies have confirmed that SOCS2 is a major negative regulator of the growth hormone (GH) signaling pathway, and SOCS2 knockout mice exhibit a pronounced overgrowth (gigantism) phenotype, with body weight, body length, and bone length all increased by approximately 30–50%. In addition to the growth hormone receptor, SOCS2 can also target multiple substrates such as the erythropoietin receptor, leptin receptor, epidermal growth factor receptor, and insulin-like growth factor-1 receptor. SOCS2 is widely expressed in various human tissues and, beyond regulating growth, also participates in numerous physiological processes including central nervous system function, metabolic regulation, immune responses, and mammary gland development. Recent studies have found that SOCS2 is abnormally expressed in various tumors; for example, in hepatocellular carcinoma, SOCS2 expression is downregulated, and its overexpression can inhibit tumor cell proliferation, migration, and invasion, whereas in pancreatic cancer, upregulated SOCS2 can promote mitochondrial dysfunction and ferroptosis. Therefore, SOCS2 is considered an important molecule linking cytokine signaling regulation with tumor initiation and progression, holding potential value as a diagnostic and therapeutic target.

Picture

Western Blot

WB result of SOCS2 Recombinant Rabbit mAb
Primary antibody: SOCS2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: K-562whole cell lysate 20 µg
Lane 3: A-204 whole cell lysate 20 µg
Lane 4: KU812 whole cell lysate 20 µg
Lane 5: Caco-2 whole cell lysate 20 µg
Negative control: Raji whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 26 kDa
This blot was developed with high sensitivity substrate

WB result of SOCS2 Recombinant Rabbit mAb
Primary antibody: SOCS2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 27 kDa
This blot was developed with high sensitivity substrate

WB result of SOCS2 Recombinant Rabbit mAb
Primary antibody: SOCS2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 27 kDa
This blot was developed with high sensitivity substrate

WB result of SOCS2 Recombinant Rabbit mAb
Primary antibody: SOCS2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 27 kDa
This blot was developed with high sensitivity substrate

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human kidney. Anti-SOCS2 antibody was used at 1/299 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human placenta. Anti-SOCS2 antibody was used at 1/299 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver cancer. Anti-SOCS2 antibody was used at 1/299 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.