WB result of SNAP29 Recombinant Rabbit mAb
Primary antibody: SNAP29 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 29 kDa
SNAP29 Recombinant Rabbit mAb (S-4163-52)
SNAP29 Recombinant Rabbit mAb (S-4163-52)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SNAP29 |
| Synonyms | Synaptosomal-associated protein 29; Golgi SNARE of 32 kDa (Gs32); Soluble 29 kDa NSF attachment protein; Vesicle-membrane fusion protein SNAP-29; Snap29 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cell membrane |
| Accession | Q9ERB0 |
| Clone Number | S-4163-52 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:1000 | Ms, Rt |
| ICC | 1:500 | Ms |
Background
SNAP29 (synaptosomal-associated protein 29) is a SNARE protein localized to intracellular organelle membranes, encoded by the SNAP29 gene, which belongs to the SNAP25 protein family and is located on human chromosome 22q11.21. Its core function is to serve as a soluble N-ethylmaleimide-sensitive factor attachment protein receptor, participating in mediating intracellular membrane fusion events through its two SNARE motifs at the N-terminus and C-terminus, particularly in autophagy by controlling the fusion of autophagosomes with lysosomal membranes, and it is also involved in ciliogenesis and membrane fusion regulation in the secretory pathway. SNAP29 can also form specific SNARE complexes with various syntaxins, such as forming a complex with STX17 and VAMP8 to mediate autophagosome-lysosome fusion, or forming complexes with Syntaxin18 or Syntaxin5 to participate in endoplasmic reticulum-to-Golgi transport. Loss-of-function mutations in this gene lead to CEDNIK syndrome, a disease characterized by congenital neurocutaneous abnormalities, including clinical manifestations such as cerebral hypoplasia, neuropathy, ichthyosis, and palmoplantar keratoderma, while SNAP29 dysfunction is also associated with the pathogenesis of cancer, viral infections, and neurodegenerative diseases.
Picture
Picture
Western Blot
WB result of SNAP29 Recombinant Rabbit mAb
Primary antibody: SNAP29 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 29 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse brain. Anti-SNAP29 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat brain. Anti-SNAP29 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in Neuro-2a cells. Anti-SNAP29 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
