WB result of Snail Recombinant Rabbit mAb
Primary antibody: Snail Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HCT 116 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: SW480 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 30 kDa
This blot was developed with high sensitivity substrate
Snail Recombinant Rabbit mAb (S-3257)
Snail Recombinant Rabbit mAb (S-3257)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Snail |
| Synonyms | Zinc finger protein SNAI1; Protein snail homolog 1 (Protein sna); SNAH; SNAI1 |
| Location | Cytoplasm, Nucleus |
| Accession | O95863 |
| Clone Number | S-3257 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Mk |
| Positive Sample | HCT 116, HeLa , A549, SW480, NIH/3T3, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Mk |
Background
Snail protein, a zinc-finger transcription factor encoded by the SNAI1 gene, is a master regulator of epithelial-to-mesenchymal transition (EMT) that represses E-cadherin and other epithelial genes, thereby promoting cell motility, invasion, and stemness, and its overexpression in diverse carcinomas is strongly linked to metastasis, chemoresistance, and poor prognosis through activation of oncogenic signaling pathways such as TGF-β, Wnt/β-catenin, and NF-κB, while its activity is modulated by post-translational modifications, including phosphorylation, ubiquitination, and acetylation, making it both a biomarker and a therapeutic target in cancer biology.
Picture
Picture
Western Blot
WB result of Snail Recombinant Rabbit mAb
Primary antibody: Snail Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 32 kDa
This blot was developed with high sensitivity substrate
WB result of Snail Recombinant Rabbit mAb
Primary antibody: Snail Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 32 kDa
This blot was developed with high sensitivity substrate
