WB result of SMN2 Recombinant Rabbit mAb
Primary antibody: SMN2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 36 kDa
SMN2 Recombinant Rabbit mAb (S-4920-4)
SMN2 Recombinant Rabbit mAb (S-4920-4)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SMN2 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q16637 |
| Clone Number | S-4920-4 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | A-431, HeLa, HepG2, NIH/3T3, mouse brain, mouse testis, PC-12, rat brain, rat testis, COS-7 |
| Predicted Reactivity | Zf, Dg, Ct, CyMk |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:200 | Hu |
Background
SMN2 protein is a multifunctional protein encoded by the SMN2 gene. Its core function is to participate in the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs), making it an indispensable key factor in the pre-mRNA splicing process. The SMN2 gene is highly homologous to the SMN1 gene, with the two differing by only a single critical nucleotide (a C-to-T transition) in exon 7. This difference causes exon 7 to be extensively skipped during the splicing of SMN2 pre-mRNA, with approximately 90% of transcripts producing a truncated protein lacking exon 7 (SMNΔ7), which is unstable and functionally impaired. As a result, SMN2 can only produce a small amount of functional full-length SMN protein, which is insufficient to fully compensate for the protein deficiency caused by SMN1 loss. SMN protein is widely expressed throughout the body and, in addition to spliceosome assembly, participates in various cellular processes including RNA metabolism, cellular homeostasis, DNA repair, and autophagy. Spinal muscular atrophy (SMA) is caused by homozygous deletion or mutation of the SMN1 gene, and SMN2 copy number is inversely correlated with disease severity—the more copies present, the higher the production of functional SMN protein and the milder the patient's phenotype. Based on this, SMN2 has become a central therapeutic target for SMA, and currently approved therapies such as the antisense oligonucleotide drug nusinersen and the small molecule drug risdiplam both work by modulating SMN2 pre-mRNA splicing to promote the inclusion of exon 7, thereby increasing the production of functional SMN protein.
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Western Blot
WB result of SMN2 Recombinant Rabbit mAb
Primary antibody: SMN2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Lane 3: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 36 kDa
WB result of SMN2 Recombinant Rabbit mAb
Primary antibody: SMN2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Lane 3: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 36 kDa
WB result of SMN2 Recombinant Rabbit mAb
Primary antibody: SMN2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 36 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human brain. Anti-SMN2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-SMN2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-SMN2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-SMN2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
