WB result of SMC1 Recombinant Rabbit mAb
Primary antibody: SMC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 143 kDa
Observed MW: 150 kDa
SMC1 Recombinant Rabbit mAb (S-4147-54)
SMC1 Recombinant Rabbit mAb (S-4147-54)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SMC1 |
| Synonyms | Structural maintenance of chromosomes protein 1A; SMC protein 1A; SMC-1-alpha; SMC-1AAlternative nameSb1.8; DXS423E; KIAA0178; SB1.8; SMC1L1; SMC1A |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q14683 |
| Clone Number | S-4147-54 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, Jurkat, HepG2, HEK-293, NIH/3T3, PC-12, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
SMC1 (Structural Maintenance of Chromosomes 1) is a highly conserved ATPase subunit of the cohesin complex, which plays a pivotal role in maintaining chromosomal architecture and ensuring genomic stability in eukaryotic cells. As a core component of the ring-shaped cohesin structure, SMC1 dimerizes with SMC3 to form the structural backbone that topologically entraps sister chromatids, thereby facilitating sister chromatid cohesion from S-phase until anaphase onset to guarantee accurate chromosome segregation during cell division. Beyond its canonical function in mitosis and meiosis, SMC1 is critically involved in DNA double-strand break repair via homologous recombination, regulation of gene expression through chromatin looping, and the establishment of proper three-dimensional genome organization. Mutations or dysregulation of SMC1 are associated with severe developmental disorders known as cohesinopathies, such as Cornelia de Lange syndrome, and are also implicated in various cancers, highlighting its essential nature in both cellular physiology and human disease pathology.
Picture
Picture
Western Blot
WB result of SMC1 Recombinant Rabbit mAb
Primary antibody: SMC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 143 kDa
Observed MW: 150 kDa
WB result of SMC1 Recombinant Rabbit mAb
Primary antibody: SMC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 143 kDa
Observed MW: 150 kDa
WB result of SMC1 Recombinant Rabbit mAb
Primary antibody: SMC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 143 kDa
Observed MW: 150 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-SMC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-SMC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-SMC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat brain. Anti-SMC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- SMC1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
