WB result of SMARCAL1 Recombinant Rabbit mAb
Primary antibody: SMARCAL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: A431 whole cell lysate 20 µg
Lane 5: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 106 kDa
Observed MW: 90-120 kDa
SMARCAL1 Recombinant Rabbit mAb (S-2110-328)
SMARCAL1 Recombinant Rabbit mAb (S-2110-328)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SMARCAL1 |
| Synonyms | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1; HepA-related protein (hHARP); Sucrose nonfermenting protein 2-like 1; HARP s |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q9NZC9 |
| Clone Number | S-2110-328 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HEK-293, A549, HeLa, A431, Jurkat, NIH/3T3, PC-12, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:50 | Hu, Ms |
Background
SMARCAL1 (SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like 1) is a DNA-stimulated ATPase and annealing helicase that travels with elongating replication forks and, upon detecting replication stress or DNA damage, catalyzes fork regression to form a protective “chicken-foot” structure and subsequent fork restoration, thereby stabilizing stalled forks, promoting template switching and homology-directed repair, and preventing MUS81-mediated double-strand breaks, while its tight regulation by RPA binding orientation and ATR-dependent phosphorylation of Ser-652 ensures controlled remodeling activity essential for maintaining genome stability, and germline mutations that abolish these functions cause the multisystem disorder Schimke immuno-osseous dysplasia.
Picture
Picture
Western Blot
WB result of SMARCAL1 Recombinant Rabbit mAb
Primary antibody: SMARCAL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 106 kDa
Observed MW: 90-120 kDa
WB result of SMARCAL1 Recombinant Rabbit mAb
Primary antibody: SMARCAL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 106 kDa
Observed MW: 90-120 kDa
WB result of SMARCAL1 Recombinant Rabbit mAb
Primary antibody: SMARCAL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 106 kDa
Observed MW: 90-150 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-SMARCAL1 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-SMARCAL1 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-SMARCAL1 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
