WB result of SMARCA3 Recombinant Rabbit mAb
Primary antibody: SMARCA3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COLO205 whole cell lysate 20 µg
Lane 2: HCT 116 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Low expression control: COLO205 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 114 kDa
Observed MW: 140 kDa
SMARCA3 Recombinant Rabbit mAb (S-3575-154)
SMARCA3 Recombinant Rabbit mAb (S-3575-154)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SMARCA3 |
| Synonyms | DNA-dependent ATPase/E3 ubiquitin-protein ligase HLTF; DNA-binding protein/plasminogen activator inhibitor 1 regulator; HIP116; Helicase-like transcription factor (HLTF); RING finger protein 80; RING-type E3 ubiquitin transferase HLTF; HIP116A; RNF80; SNF2L3; ZBU1; HLTF |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q14527 |
| Clone Number | S-3575-154 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HCT 116, HeLa, K562, NIH/3T3, C6, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:500 | Hu, Ms, Rt |
| ICC | 1:100 | Hu, Ms, Rt |
Background
SMARCA3, also known as HLTF (helicase-like transcription factor), is a member of the SWI/SNF chromatin remodeling family. However, unlike traditional remodeling complexes, it functions independently through its unique dual enzymatic activities—ATP-dependent helicase-like activity and E3 ubiquitin ligase activity. Its functions are multifaceted: in DNA replication and damage response, it catalyzes K63-linked polyubiquitination of PCNA protein, promoting replication fork reversal to repair damaged DNA; simultaneously, it also serves as a specific E3 ubiquitin ligase for histone H3K23, stabilizing chromatin structure by maintaining the repressive histone modification H3K9me3. SMARCA3 is widely expressed in various tissues, particularly in the nervous system where it binds to the p11/annexin A2 complex and participates in regulating behavioral responses to antidepressant drugs. Its dysregulated expression is closely associated with tumors: in colorectal cancer, approximately 40% of cases exhibit SMARCA3 silencing due to promoter hypermethylation of the HLTF gene, which is associated with chromatin opening, oncogene activation, and tumor progression, establishing its important role as a tumor suppressor.
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Western Blot
WB result of SMARCA3 Recombinant Rabbit mAb
Primary antibody: SMARCA3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 114 kDa
Observed MW: 140 kDa
WB result of SMARCA3 Recombinant Rabbit mAb
Primary antibody: SMARCA3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 114 kDa
Observed MW: 140 kDa
WB result of SMARCA3 Recombinant Rabbit mAb
Primary antibody: SMARCA3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 114 kDa
Observed MW: 140 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-SMARCA3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-SMARCA3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-SMARCA3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-SMARCA3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-SMARCA3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-SMARCA3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in K562 cells. Anti- SMARCA3 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- SMARCA3 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in C6 cells. Anti- SMARCA3 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
