IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-SLC8A1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
SLC8A1 Recombinant Rabbit mAb (S-3100-17)
SLC8A1 Recombinant Rabbit mAb (S-3100-17)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SLC8A1 |
| Synonyms | Sodium/calcium exchanger 1; Na(+)/Ca(2+)-exchange protein 1; Solute carrier family 8 member 1; CNC; NCX1 s |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P32418 |
| Clone Number | S-3100-17 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P, IF |
| Reactivity | Rt |
| Predicted Reactivity | GP, Dg, Bv, Ct |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:500 | Rt |
| IF | 1:500 | Rt |
Background
SLC8A1, also known as NCX1, is a 70-kDa Na⁺/Ca²⁺ exchanger that resides primarily in the sarcolemma of cardiomyocytes, where it catalyzes the electrogenic counter-transport of 3 Na⁺ for 1 Ca²⁺ and thus functions as the dominant Ca²⁺ efflux pathway during diastole; its activity is allosterically regulated by intracellular Ca²⁺, inhibited by intracellular Na⁺ and the lipid PIP₂, and its dysfunction or overexpression contributes to arrhythmias, heart failure, and hypertension, while the gene contains multiple promoters giving rise to tissue-specific splice variants that differ only in their short regulatory N-terminal regions.
Picture
Picture
Immunohistochemistry
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-SLC8A1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-SLC8A1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded rat kidney. Anti-SLC8A1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
