IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-SLC6A1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
SLC6A1 Recombinant Rabbit mAb (SDT-2424-278)
SLC6A1 Recombinant Rabbit mAb (SDT-2424-278)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SLC6A1 |
| Synonyms | Sodium- and chloride-dependent GABA transporter 1; GAT-1; Solute carrier family 6 member 1; GABATR; GABT1; GAT1 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P30531 |
| Clone Number | SDT-2424-278 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| IF | 1:500 | Rt |
Background
SLC6A1 encodes GAT-1, the principal Na⁺-coupled transporter that clears the inhibitory neurotransmitter GABA from the synaptic cleft by pumping it back into neurons and astrocytes, thereby terminating GABAergic signaling and maintaining excitatory–inhibitory balance throughout the CNS; mutations that misfold GAT-1 or abolish its plasma-membrane trafficking reduce synaptic GABA clearance, causing epilepsy, intellectual disability, autism spectrum disorders, ADHD, and mood dysfunction, whereas chemical or pharmacological chaperones can rescue some variants by restoring correct folding and surface expression.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebellum. Anti-SLC6A1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-SLC6A1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebellum. Anti-SLC6A1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-SLC6A1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded rat retina. Anti-SLC6A1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
