WB result of SLC25A22 Recombinant Rabbit mAb
Primary antibody: SLC25A22 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PANC-1 whole cell lysate 20 µg
Lane 2: HT-29 whole cell lysate 20 µg
Lane 3: Caco-2 whole cell lysate 20 µg
Lane 4: A431 whole cell lysate 20 µg
Lane 5: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 30 kDa
SLC25A22 Recombinant Rabbit mAb (S-2780-15)
SLC25A22 Recombinant Rabbit mAb (S-2780-15)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SLC25A22 |
| Synonyms | Mitochondrial glutamate carrier 1; GC-1; Glutamate/H(+) symporter 1; Solute carrier family 25 member 22; GC1 |
| Immunogen | Recombinant Protein |
| Location | Mitochondrion |
| Accession | Q9H936 |
| Clone Number | S-2780-15 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | PANC-1, HT-29, Caco-2, A431, HepG2, NIH/3T3, mouse brain, PC-12, rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt |
| IHC-P | 1:100 | Hu |
| ICC | 1:100 | Hu |
Background
SLC25A22 is a mitochondrial inner-membrane carrier that exchanges glutamate for H+ across the membrane, thereby providing the matrix with the key anaplerotic substrate needed to sustain the TCA cycle and amino-acid metabolism in neurons and β-cells; mutations in this gene abolish transport activity, cause autosomal-recessive early infantile epileptic encephalopathy-3 (EIEE3) with suppression-burst patterns, and are emerging as drivers of certain cancers by disrupting redox and metabolic homeostasis.
Picture
Picture
Western Blot
WB result of SLC25A22 Recombinant Rabbit mAb
Primary antibody: SLC25A22 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 30 kDa
WB result of SLC25A22 Recombinant Rabbit mAb
Primary antibody: SLC25A22 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 30 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-SLC25A22 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-SLC25A22 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-SLC25A22 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HT-29 cells. Anti- SLC25A22 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
