WB result of SLC12A2 Recombinant Rabbit mAb
Primary antibody: SLC12A2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HT-29 whole cell lysate 20 µg
Lane 3: COLO 205 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 131 kDa
Observed MW: 120-220 kDa
SLC12A2 Recombinant Rabbit mAb (S-2437-30)
SLC12A2 Recombinant Rabbit mAb (S-2437-30)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SLC12A2 |
| Synonyms | Solute carrier family 12 member 2; Basolateral Na-K-Cl symporter; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2 (BSC2); Na-K-2Cl cotransporter 1 (hNKCC1); NKCC1 s |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P55011 |
| Clone Number | S-2437-30 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | MCF7, HT-29, COLO 205, NIH/3T3, mouse brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt |
| IHC-P | 1:200 | Hu, Ms |
Background
SLC12A2 (solute carrier family 12 member 2), also known as NKCC1 (Na⁺-K⁺-2Cl⁻ cotransporter-1), is a 1,215-residue, bumetanide-sensitive, electromeutral plasma-membrane protein that uses the inward Na⁺ gradient generated by Na⁺/K⁺-ATPase to drive uphill import of K⁺ and Cl⁻ into cells, thereby regulating ionic homeostasis, cell volume, epithelial salt movement, blood pressure, hearing, GABAergic inhibition, and neuronal excitability through dynamic phosphorylation by oxidative-stress-responsive kinases (e.g., SPAK/OSR1) and membrane trafficking in epithelia, neurons, vascular smooth muscle, immune cells, and secretory glands, with pathophysiological relevance to hypertension, deafness, neuropathic pain, and tumor cell proliferation.
Picture
Picture
Western Blot
WB result of SLC12A2 Recombinant Rabbit mAb
Primary antibody: SLC12A2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Negative control: mouse liver lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 131 kDa
Observed MW: 180-220 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-SLC12A2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-SLC12A2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-SLC12A2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
