WB result of SKP2 Recombinant Rabbit mAb
Primary antibody: SKP2 Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: Hep G2 whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: K-562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 40, 48 kDa
SKP2 Recombinant Rabbit mAb
SKP2 Recombinant Rabbit mAb
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SKP2 |
| Synonyms | S-phase kinase-associated protein 2; Cyclin-A/CDK2-associated protein p45; F-box protein Skp2; F-box/LRR-repeat protein 1; p45skp2; FBXL1 |
| Location | Cytoplasm, Nucleus |
| Accession | Q13309 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu |
| Positive Sample | HepG2, HEK-293, Jurkat, K-562 |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000-1:20000 | Hu |
| ICC | 1:500 | Hu |
Picture
Picture
Western Blot
Immunocytochemistry
ICC shows positive staining in HepG2 cells. Anti-SKP2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
