Skp2 Recombinant Rabbit mAb (S-4906)

Catalog Number: S0B6879 Application: WB, IHC-P, ICC Reactivity: Hu, Mk Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25µl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	SKP2
Synonyms	S-phase kinase-associated protein 2; Cyclin-A/CDK2-associated protein p45; F-box protein Skp2; F-box/LRR-repeat protein 1; p45skp2; FBXL1
Location	Nucleus
Accession	Q13309
Clone Number	S-4906
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P, ICC
Reactivity	Hu, Mk
Positive Sample	HeLa, U-2 OS, LNCap, K562, U-87 MG, SK-OV-3, MCF7, COS-7
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Mk
IHC-P	1:100	Hu
ICC	1:500	Hu

Background

Skp2 (S-phase kinase-associated protein 2) is an F-box protein that serves as the substrate recognition component of the SCF (Skp1-Cul1-F-box) E3 ubiquitin ligase complex, playing a pivotal role in regulating cell cycle progression, particularly G1/S transition, through targeted proteolysis of key cell cycle regulators. As an oncoprotein frequently overexpressed in various human cancers, Skp2 specifically recognizes and promotes the ubiquitination and subsequent degradation of critical tumor suppressors including p27Kip1, p21Cip1, p57Kip2, and FOXO1, thereby driving cellular proliferation and inhibiting apoptosis. Beyond its canonical function in cell cycle control, Skp2 has emerged as a multifaceted regulator involved in DNA replication, metabolism, autophagy, and cancer metastasis through interactions with diverse substrates such as cyclin E, c-Myc, and Akt. The aberrant elevation of Skp2 expression, often resulting from genomic amplification, transcriptional upregulation, or impaired degradation, correlates with poor prognosis, tumor aggressiveness, and therapeutic resistance across multiple malignancies including breast, prostate, and lung cancers, making Skp2 an attractive therapeutic target for cancer intervention.

Picture

Western Blot

WB result of Skp2 Recombinant Rabbit mAb
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Skp2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: U-2 OS whole cell lysate 20 µg
Lane 3: LNCaP whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Lane 5: U-87 MG whole cell lysate 20 µg
Lane 6: SK-OV-3 whole cell lysate 20 µg
Lane 7: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 48 kDa

WB result of Skp2 Recombinant Rabbit mAb
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Skp2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 48 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon. Anti-Skp2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human tonsil. Anti-Skp2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Skp2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in HeLa cells. Anti-Skp2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).