WB result of SIRT2 Mouse mAb
Primary antibody: SIRT2 Mouse mAb at 1/5000 dilution
Lane 1: HEK293 whole cell lysate 20 µg
Lane 2: LNCaP whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 37-40 kDa
This blot was developed with high sensitivity substrate
SIRT2 Mouse mAb
SIRT2 Mouse mAb
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | SIRT2 |
| Synonyms | NAD-dependent protein deacetylase sirtuin-2; NAD-dependent protein deacylase sirtuin-2; NAD-dependent protein defatty-acylase sirtuin-2; Regulatory protein SIR2 homolog 2; SIR2-like protein 2; SIR2L; SIR2L2; SIRT2 |
| Location | Cytoplasm, Cytoskeleton, Nucleus, Cell membrane |
| Accession | Q8IXJ6 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1 |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HEK293, LNCaP, mouse brain, rat brain |
| Purification | Protein G |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000 | Hu, Ms, Rt |
| IHC-P | 1:50 | Hu, Ms, Rt |
Background
SIRT2 (Sirtuin 2) is a primarily cytoplasmic, NAD+-dependent protein deacetylase that regulates critical cellular processes such as the cell cycle, metabolism, and autophagy. As a key upstream regulator of the mitotic surveillance protein BubR1, SIRT2 helps maintain genomic stability and prevent aneuploidy, making it closely linked to aging and cancer development. It exhibits context-dependent roles in tumorigenesis (acting as either an oncogene or tumor suppressor) and is currently being investigated as a potential therapeutic target for age-related disorders, including non-small cell lung cancer and neurodegenerative diseases.
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Western Blot
WB result of SIRT2 Mouse mAb
Primary antibody: SIRT2 Mouse mAb at 1/5000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 37-40 kDa
WB result of SIRT2 Mouse mAb
Primary antibody: SIRT2 Mouse mAb at 1/5000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 37-40 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-SIRT2 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-SIRT2 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-SIRT2 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-SIRT2 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-SIRT2 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
