WB result of SIGMAR1 Recombinant Rabbit mAb
Primary antibody: SIGMAR1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: Caco-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 28 kDa
SIGMAR1 Recombinant Rabbit mAb (S-2079-270)
SIGMAR1 Recombinant Rabbit mAb (S-2079-270)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SIGMAR1 |
| Synonyms | Sigma non-opioid intracellular receptor 1; Aging-associated gene 8 protein; SR31747-binding protein (SR-BP); Sigma 1-type opioid receptor (SIG-1R; Sigma1-receptor; Sigma1R; hSigmaR1); OPRS1; SRBP |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane, Nucleus membrane, Endoplasmic reticulum membrane |
| Accession | Q99720 |
| Clone Number | S-2079-270 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, 293T, Caco-2, NIH/3T3, mouse liver, mouse brain, C6, rat liver, rat brain, COS-7 |
| Predicted Reactivity | Bv, GP, Ft |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt, Mk |
| ICC | 1:100 | Hu, Ms |
| FCM | e |
Background
The SIGMAR1 protein, also known as the sigma-1 receptor, is a multifunctional chaperone protein composed of 223 amino acids with a molecular mass of 25.3 kDa. It is encoded by the SIGMAR1 gene and is ubiquitously expressed in various tissues, including the brain, heart, liver, and kidneys. SIGMAR1 is primarily located in the mitochondria-associated endoplasmic reticulum membrane (MAM), where it regulates calcium signaling, ion channel activity, and mitochondrial function. This protein plays a crucial role in cellular survival and has been implicated in several diseases, such as neurodegenerative disorders (e.g., amyotrophic lateral sclerosis and Alzheimer's disease), cardiovascular diseases, and certain cancers. Additionally, SIGMAR1 has been shown to have protective effects against neuronal and cardiac injuries.
Picture
Picture
Western Blot
WB result of SIGMAR1 Recombinant Rabbit mAb
Primary antibody: SIGMAR1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 28 kDa
WB result of SIGMAR1 Recombinant Rabbit mAb
Primary antibody: SIGMAR1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat liver lysate 20 µg
Lane 3: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 28 kDa
WB result of SIGMAR1 Recombinant Rabbit mAb
Primary antibody: SIGMAR1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 28 kDa
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- SIGMAR1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- SIGMAR1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
