WB result of Siglec-8 Recombinant Rabbit mAb
Primary antibody: Siglec-8 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector (vector control) containing a myc-His-tag whole cell Lysate 20 µg
Lane 2: 293T cells transfected with a human SIGLEC8 expression vector containing a myc-His-tag whole cell Lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 62 kDa
Siglec-8 Recombinant Rabbit mAb (S-2049-19)
Siglec-8 Recombinant Rabbit mAb (S-2049-19)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Siglec-8 |
| Synonyms | Sialic acid-binding Ig-like lectin 8; Sialoadhesin family member 2 (SAF-2); SAF2; SIGLEC8 |
| Immunogen | Synthetic Peptide |
| Location | Membrane |
| Accession | Q9NYZ4 |
| Clone Number | S-2049-19 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu |
| ICC | 1:500 | Hu |
Background
Siglec-8 is a CD33-related cell-surface lectin selectively expressed on human eosinophils and, at lower density, on mast cells and basophils; it carries an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an ITIM-like motif that, upon engagement by sialylated glycan ligands, recruit SHP-1/2 phosphatases to deliver a potent inhibitory signal that suppresses FcεRI- or cytokine-driven activation, survival and mediator release, thereby serving as a key “off-switch” for allergic inflammation and making it an attractive therapeutic target for eosinophilic asthma, chronic urticaria and other IgE-mediated diseases.
Picture
Picture
Western Blot
Immunocytochemistry
ICC shows positive staining in Myc-His transfected 293F cells (top panel) and negative staining in vector-transfected 293F cells (below panel). Anti- Siglec-8 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
