WB result of Shh Recombinant Rabbit mAb
Primary antibody: Shh Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: LnCap whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 52 kDa
Shh Recombinant Rabbit mAb
Shh Recombinant Rabbit mAb
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Shh |
| Synonyms | Sonic hedgehog protein; HHG-1; Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains (ShhNC); SHH |
| Location | Secreted, Cell membrane, Endoplasmic reticulum |
| Accession | Q15465 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HepG2, LnCap, C2C12, mouse liver, rat liver |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000-1:50000 | Hu, Ms, Rt |
Background
Sonic Hedgehog (Shh) is a crucial secreted signaling protein and the most well-studied member of the Hedgehog family, playing an irreplaceable role in embryonic development. It acts as a morphogen to govern the dorsoventral patterning of the neural tube, the anteroposterior arrangement of limbs, and the formation of central nervous system structures. Upon binding to the Patched (Ptc) receptor on target cells, Shh relieves the inhibition of Smoothened (Smo), triggering an intracellular signaling cascade that activates Gli transcription factors to regulate specific gene expression. Beyond its developmental functions, Shh is essential for tissue maintenance and regeneration, and its dysregulated signaling is frequently implicated in various human cancers and severe congenital defects, such as holoprosencephaly.
Picture
Picture
Western Blot
WB result of Shh Recombinant Rabbit mAb
Primary antibody: Shh Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 52 kDa
WB result of Shh Recombinant Rabbit mAb
Primary antibody: Shh Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: rat liver lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 52 kDa
