WB result of SFRP1 Recombinant Rabbit mAb
Primary antibody: SFRP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: SK-MEL-28 whole cell lysate 20 µg
Lane 4: HEK-293 whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate
Low expression control: HEK-293 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 35 kDa
SFRP1 Recombinant Rabbit mAb (S-3071-18)
SFRP1 Recombinant Rabbit mAb (S-3071-18)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SFRP1 |
| Synonyms | Secreted frizzled-related protein 1; FRP-1; sFRP-1; Secreted apoptosis-related protein 2 (SARP-2); FRP; FRP1; SARP2 |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | Q8N474 |
| Clone Number | S-3071-18 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | A549, SK-MEL-28 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:200 | Hu |
Background
SFRP1 (Secreted Frizzled-Related Protein 1) is a 313-amino-acid, cysteine-rich antagonist of Wnt signaling that is secreted into the extracellular space where it binds Wnt ligands and Frizzled receptors to modulate β-catenin–dependent and –independent pathways, thereby regulating embryonic segmentation, eye, heart and bone development, as well as adult tissue homeostasis, while its epigenetic or mutational silencing in cancers removes this brake on Wnt signaling and drives tumor progression, invasion and metastasis.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-SFRP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-SFRP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-SFRP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-SFRP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
