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SEZ6 Recombinant Rabbit mAb (SDT-2097-737)

SEZ6 Recombinant Rabbit mAb (SDT-2097-737)

Catalog Number: S0B2448 Application: WB, IHC-P Reactivity: Human Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Rabbit
Antigen SEZ6
Synonyms Seizure protein 6 homolog; SEZ-6; hSEZ-6
Immunogen Recombinant Protein
Location Cell membrane
Accession Q53EL9
Clone Number SDT-2097-737
Antibody Type Recombinant mAb
Isotype IgG
Application WB, IHC-P
Reactivity Hu
Positive Sample H69
Purification Protein A
Concentration 1 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:1000-1:10000 Hu
IHC-P 1:2000 Hu

Background

SEZ6 (Seizure-related gene 6) is a type I transmembrane glycoprotein (~110 kDa) composed of five short consensus repeat (SCR) domains, three CUB domains, a single transmembrane segment and a short cytoplasmic tail that is predominantly expressed in the central nervous system, where it acts as a critical trafficking chaperone for kainate receptor subunits GluK2/3, ensuring their proper N-glycosylation (including the HNK-1 epitope), post-ER export and surface localization to regulate synaptic connectivity, long-term potentiation and dendritic arbor balance, and is sequentially cleaved by BACE1 and γ-secretase to release a soluble ectodomain whose CSF level serves as a direct pharmacodynamic read-out of BACE1 inhibition, while in neuroendocrine neoplasms its selective over-expression is exploited as an ADC target for therapies against small-cell lung cancer and related tumors.

Picture

Western Blot

WB result of SEZ6 Recombinant Rabbit mAb
Primary antibody: SEZ6 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: H69 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 107 kDa
Observed MW: 130-200 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-SEZ6 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cerebellum. Anti-SEZ6 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human small cell lung cancer. Anti-SEZ6 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human lung squamous cell carcinoma. Anti-SEZ6 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human lung adenocarcinoma. Anti-SEZ6 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.