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SERPINB3 Recombinant Rabbit mAb (S-4587)

SERPINB3 Recombinant Rabbit mAb (S-4587)

Catalog Number: S0B6767 Application: WB, IHC-P Reactivity: Human Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Rabbit
Antigen SERPINB3
Synonyms Serpin B3; Protein T4-A; Squamous cell carcinoma antigen 1 (SCCA-1); SCCA; SCCA1
Location Cytoplasm, Secreted
Accession P29508
Clone Number S-4587
Antibody Type Recombinant mAb
Isotype IgG
Application WB, IHC-P
Reactivity Hu
Positive Sample A431, BxPC-3
Purification Protein A
Concentration 0.5 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:1000 Hu
IHC-P 1:2000 Hu

Background

SERPINB3, also known as Squamous Cell Carcinoma Antigen 1 (SCCA1), is a member of the clade B serpin family that functions primarily as an intracellular inhibitor of cysteine and serine proteases, playing a critical role in regulating apoptosis and protecting cells from protease-induced damage. Normally expressed in stratified squamous epithelia such as the skin, cervix, and lung, this protein helps maintain tissue integrity by inhibiting enzymes like cathepsins and papain-like proteases; however, its expression is frequently and significantly upregulated in various malignancies, particularly squamous cell carcinomas of the head and neck, lung, esophagus, and cervix, where it contributes to tumor progression by conferring resistance to chemotherapy and radiation through the suppression of granzyme B-mediated cytotoxicity and the inhibition of Fas-mediated apoptotic pathways. Due to its strong association with advanced disease stages, poor prognosis, and therapeutic resistance, SERPINB3 serves as a valuable clinical biomarker for monitoring tumor burden and recurrence, while also emerging as a potential therapeutic target aimed at restoring apoptotic sensitivity in resistant cancer cells.

Picture

Western Blot

WB result of SERPINB3 Recombinant Rabbit mAb
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: SERPINB3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: IMR-32 whole cell lysate 20 µg
Lane 2: HL-60 whole cell lysate 20 µg 
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: A431 whole cell lysate 20 µg
Lane 5: BxPC-3 whole cell lysate 20 µg
Negative control: IMR-32 whole cell lysate; HL-60 whole cell lysate; HepG2 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45 kDa
Observed MW: 45 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human esophagus. Anti-SERPINB3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-SERPINB3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervical squamous cell carcainoma. Anti-SERPINB3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-SERPINB3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.