WB result of SERCA2 ATPase Recombinant Rabbit mAb
Primary antibody: SERCA2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled HeLa whole cell lysate 20 µg
Lane 2: unboiled HepG2 whole cell lysate 20 µg
Lane 3: unboiled Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 115 kDa
Observed MW: 115 kDa
SERCA2 ATPase Recombinant Rabbit mAb (S-5956)
SERCA2 ATPase Recombinant Rabbit mAb (S-5956)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SERCA2 ATPase |
| Location | Endoplasmic reticulum |
| Accession | P16615 |
| Clone Number | S-5956 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, HepG2, Jurkat, C2C12, NIH/3T3, mouse brain, PC-12, rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:2000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms |
Background
SERCA2 ATPase, or Sarco/Endoplasmic Reticulum Calcium ATPase 2, is a key member of the P-type ATPase family encoded by the ATP2A2 gene, primarily responsible for actively pumping calcium ions from the cytoplasm into the lumen of the sarcoplasmic or endoplasmic reticulum, thereby maintaining intracellular calcium homeostasis and driving various physiological processes such as muscle relaxation. The protein contains three major structural domains: the N-domain responsible for ATP binding, the P-domain that accepts phosphoryl transfer, and the A-domain involved in calcium translocation. Through the formation of phosphorylated intermediates (E1~P and E2~P conformations), it achieves efficient transport of two calcium ions per ATP molecule hydrolyzed against a steep concentration gradient. Physiologically, the SERCA2a isoform is the predominant isoform in cardiac and smooth muscle, with its activity dynamically regulated by endogenous factors such as phospholamban (PLB), while the SERCA2b isoform is broadly expressed in the endoplasmic reticulum of non-muscle cells and is closely associated with cellular secretory functions. At the pathological level, mutations in the ATP2A2 gene are the underlying cause of Darier's disease, a rare autosomal dominant genodermatosis, and its functional impairment is also closely linked to major diseases including heart failure, diabetic cardiomyopathy, Alzheimer's disease, and various cancers—in heart failure, downregulation or insufficient activity of SERCA2a leads to diastolic dysfunction, while in certain tumors its aberrant expression is involved in regulating the balance between cell proliferation and apoptosis. Therefore, strategies that modulate SERCA2 ATPase activity or expression levels, such as gene therapy and development of small-molecule agonists, have become important research directions for treating cardiovascular diseases and counteracting other related pathological conditions.
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Western Blot
WB result of SERCA2 ATPase Recombinant Rabbit mAb
Primary antibody: SERCA2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled C2C12 whole cell lysate 20 µg
Lane 2: unboiled NIH/3T3 whole cell lysate 20 µg
Lane 3: unboiled mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 115 kDa
Observed MW: 115 kDa
WB result of SERCA2 ATPase Recombinant Rabbit mAb
Primary antibody: SERCA2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled PC-12 whole cell lysate 20 µg
Lane 2: unboiled rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 115 kDa
Observed MW: 115 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human brain. Anti-SERCA2 ATPase antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-SERCA2 ATPase antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-SERCA2 ATPase antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-SERCA2 ATPase antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical cancer. Anti-SERCA2 ATPase antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-SERCA2 ATPase antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse brain. Anti-SERCA2 ATPase antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat brain. Anti-SERCA2 ATPase antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-SERCA2 ATPase antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti-SERCA2 ATPase antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
