Sec61B Rabbit Polyclonal Antibody

Catalog Number: S0B6946 Application: WB, IHC-P Reactivity: Hu, Mk Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	Sec61B
Synonyms	Protein transport protein Sec61 subunit beta; SEC61B
Immunogen	Synthetic Peptide
Location	Endoplasmic reticulum
Accession	P60468
Antibody Type	Polyclonal antibody
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Mk
Positive Sample	293T, HeLa, HepG2, A431, C2C12, COS-7
Predicted Reactivity	Dg
Purification	Immunogen Affinity
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:2000	Hu, Mk
IHC-P	1:1000	Hu

Background

Sec61B is a critical subunit of the Sec61 translocon complex, which serves as the primary protein-conducting channel in the endoplasmic reticulum (ER) membrane of eukaryotic cells. As part of the heterotrimeric Sec61 complex—composed of Sec61α, Sec61β (Sec61B), and Sec61γ—Sec61B plays an essential structural and regulatory role in facilitating the co-translational translocation of nascent polypeptide chains into the ER lumen or their integration into the ER membrane. Although it is the smallest subunit, Sec61B stabilizes the complex by interacting with Sec61α and helps maintain the integrity of the translocation pore, thereby ensuring efficient protein secretion and membrane protein biogenesis. Beyond its canonical role in protein translocation, emerging evidence suggests that Sec61B may also participate in ER stress responses, calcium homeostasis, and the regulation of channel gating, highlighting its multifaceted importance in cellular proteostasis and overall ER function.

Picture

Western Blot

WB result of Sec61B Rabbit pAb
Primary antibody: Sec61B Rabbit pAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 10 kDa
Observed MW: 13 kDa

WB result of Sec61B Rabbit pAb
Primary antibody: Sec61B Rabbit pAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 10 kDa
Observed MW: 13 kDa

WB result of Sec61B Rabbit pAb
Primary antibody: Sec61B Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 10 kDa
Observed MW: 13 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human kidney. Anti-Sec61B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-Sec61B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.