SEC24D Rabbit Polyclonal Antibody

Catalog Number: S0B60546 Application: WB, IHC-P Reactivity: Hu, Ms, Rt, Mk Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	SEC24D
Immunogen	Synthetic Peptide
Location	Cytoplasm, Endoplasmic reticulum
Accession	O94855
Antibody Type	Polyclonal antibody
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt, Mk
Positive Sample	MCF7, HeLa, SK-OV-3, SH-SY5Y, A549, NIH/3T3, RAW264.7, PC-12, COS-7
Purification	Immunogen Affinity
Concentration	2 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied.

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt, Mk
IHC-P	1:500-1:2000	Hu

Background

SEC24D (SEC24 homolog D) is a key cargo-selection subunit of the COPII vesicle coat, encoded by the SEC24D gene located on chromosome 4q26, with a protein of 1,032 amino acids and a molecular weight of approximately 115 kDa. Structurally, SEC24D contains a conserved zinc finger domain, a Sec23/Sec24 trunk domain, a β-sandwich domain, a helical domain, and a gelsolin-like domain, forming multiple cargo-binding sites including an IxM-binding pocket. Its core function is to form a heterodimer with SEC23 at endoplasmic reticulum exit sites, selectively packaging transmembrane cargo proteins into COPII vesicles by recognizing specific motifs in their cytoplasmic tails, thereby mediating anterograde transport of secretory proteins from the endoplasmic reticulum to the Golgi apparatus. SEC24D exhibits unique cargo selectivity: its IxM-binding pocket can recognize cargo such as the SNARE proteins GOSR2 and STX5 that contain IxM motifs, and it can also specifically package GPI-anchored proteins through the cargo receptor TMED10. Clinically, mutations in SEC24D are closely associated with Cole-Carpenter syndrome type 2, an autosomal recessive bone formation disorder characterized by craniofacial malformations, defective skull ossification, and susceptibility to fractures. In addition, SEC24D has been identified as a susceptibility gene for hepatitis B virus infection and shows aberrant expression and functional necessity in various tumors.

Picture

Western Blot

WB result of SEC24D Rabbit Polyclonal Antibody
Primary antibody: SEC24D Rabbit Polyclonal Antibody at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: SK-OV-3 whole cell lysate 20 µg
Lane 4: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 113 kDa
Observed MW: 115 kDa

WB result of SEC24D Rabbit Polyclonal Antibody
Primary antibody: SEC24D Rabbit Polyclonal Antibody at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW 264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 113 kDa
Observed MW: 105 kDa

WB result of SEC24D Rabbit Polyclonal Antibody
Primary antibody: SEC24D Rabbit Polyclonal Antibody at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 113 kDa
Observed MW: 105 kDa

WB result of SEC24D Rabbit Polyclonal Antibody
Primary antibody: SEC24D Rabbit Polyclonal Antibody at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 113 kDa
Observed MW: 100 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human kidney. Anti-SEC24D antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-SEC24D antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-SEC24D antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.