WB result of SEC24C Rabbit Polyclonal Antibody
Primary antibody: SEC24C Rabbit Polyclonal Antibody at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: SK-OV-3 whole cell lysate 20 µg
Lane 3: HT-29 whole cell lysate 20 µg
Lane 4: K-562 whole cell lysate 20 µg
Lane 5: PC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 118 kDa
Observed MW: 125 kDa
SEC24C Rabbit Polyclonal Antibody
SEC24C Rabbit Polyclonal Antibody
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SEC24C |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Endoplasmic reticulum |
| Accession | P53992 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, SK-OV-3, HT-29, K-562, PC-3, NIH/3T3, mouse liver, mouse spleen, rat liver, rat spleen, COS-7 |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
Background
SEC24C (SEC24 homolog C, component of the COPII coat complex) is a key cargo-selection subunit of the COPII vesicle coat, encoded by the SEC24C gene located on chromosome 10q22.2, with a protein of 1,125 amino acids and a molecular weight of approximately 118 kDa. Structurally, SEC24C contains conserved zinc finger, β-sandwich, helical domains, and a gelsolin-like domain, forming multiple cargo-binding pockets. Its core function is to form a heterodimer with SEC23 at endoplasmic reticulum exit sites, selectively recognizing transmembrane cargo proteins through di-acidic or di-hydrophobic motifs in their cytoplasmic tails, concentrating them into newly formed COPII vesicles, and thereby mediating anterograde transport of secretory proteins from the endoplasmic reticulum to the Golgi apparatus. SEC24C exhibits unique cargo selectivity: its surface groove can bind IxM motifs (such as in the SNARE proteins GOSR2 and STX5), and it specifically packages GPI-anchored proteins through the cargo receptor TMED10. In addition, SEC24C is involved in HIV-1 viral replication as an important host dependency factor, and in neurons it maintains proteostasis and polarity by transporting ion channels and receptors.
Picture
Picture
Western Blot
WB result of SEC24C Rabbit Polyclonal Antibody
Primary antibody: SEC24C Rabbit Polyclonal Antibody at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 118 kDa
Observed MW: 125 kDa
WB result of SEC24C Rabbit Polyclonal Antibody
Primary antibody: SEC24C Rabbit Polyclonal Antibody at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 118 kDa
Observed MW: 115 kDa
This blot was developed with high sensitivity substrate
WB result of SEC24C Rabbit Polyclonal Antibody
Primary antibody: SEC24C Rabbit Polyclonal Antibody at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Lane 2: rat spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 118 kDa
Observed MW: 115 kDa
This blot was developed with high sensitivity substrate
WB result of SEC24C Rabbit Polyclonal Antibody
Primary antibody: SEC24C Rabbit Polyclonal Antibody at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 118 kDa
Observed MW: 110 kDa
