WB result of SEC23B Recombinant Rabbit mAb
Primary antibody: SEC23B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: Raji whole cell lysate 20 µg
Lane 3: MDA-MB-231 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Lane 5: SW620 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 86 kDa
SEC23B Recombinant Rabbit mAb (S-1935-28)
SEC23B Recombinant Rabbit mAb (S-1935-28)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SEC23B |
| Synonyms | Protein transport protein Sec23B; hSec23B; SEC23-related protein B |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Endoplasmic reticulum |
| Accession | Q15437 |
| Clone Number | S-1935-28 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | K562, Raji, MDA-MB-231, MCF7, SW620, RAW264.7, NIH/3T3, C6 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:250 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
SEC23B, also known as Sec23 homolog B, is a crucial component of the coat protein complex II (COPII), which is involved in the transport of proteins from the endoplasmic reticulum (ER) to the Golgi apparatus. This gene plays a significant role in cellular processes such as autophagy regulation and cell migration. Recent studies have shown that SEC23B can induce autophagy in breast cancer cells by inhibiting the ERK/mTOR signaling pathway, thereby promoting cancer cell metastasis. Additionally, SEC23B has been implicated in various cancers, including thyroid cancer, hepatocellular carcinoma, and prostate cancer, suggesting its potential as a therapeutic target.
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Picture
Western Blot
WB result of SEC23B Recombinant Rabbit mAb
Primary antibody: SEC23B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 86 kDa
WB result of SEC23B Recombinant Rabbit mAb
Primary antibody: SEC23B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 86 kDa
Observed MW: 86 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti- SEC23B antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti- SEC23B antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti- SEC23B antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti- SEC23B antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti- SEC23B antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti- SEC23B antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in Raji cells. Anti- SEC23B antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
