SCTR Recombinant Rabbit mAb (S-3949-52)
SCTR Recombinant Rabbit mAb (S-3949-52)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SCTR |
| Synonyms | Secretin receptor; Sctr |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | Q5FWI2 |
| Clone Number | S-3949-52 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Ms, Rt |
| Positive Sample | mouse pancreas, rat pancreas |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Ms, Rt |
| IHC-P | 1:200 | Ms, Rt |
Background
SCTR (Secretin Receptor) is a G protein-coupled receptor encoded by the SCTR gene that binds the hormone secretin. It plays a crucial role in regulating the pH of the duodenum by inhibiting gastric acid secretion and stimulating pancreatic bicarbonate production. Additionally, SCTR is involved in diet-induced thermogenesis via brown fat activation, renal water homeostasis, and synaptic plasticity in the central nervous system.
Picture
Picture
Western Blot
WB result of SCTR Recombinant Rabbit mAb
Primary antibody: SCTR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse skeletal muscle lysate 20 µg
Lane 2: mouse pancreas lysate 20 µg
Negative control: mouse skeletal muscle lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 50 kDa
WB result of SCTR Recombinant Rabbit mAb
Primary antibody: SCTR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat pancreas lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 55 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse colon. Anti-SCTR antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse skeletal muscle. Anti-SCTR antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-SCTR antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-SCTR antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat skeletal muscle. Anti-SCTR antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
